Coding

Part:BBa_K2560268:Design

Designed by: Memduha Muratoglu   Group: iGEM18_Marburg   (2018-10-01)


tfoX (V. cholerae)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 160


Design Notes

The part contains the coding region from the tfoX gene of V. cholerae without the start codon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsaI recognition sites (Weber et al., 2011). It was built with the Marburg Toolbox, a golden gate based toolbox for modular cloning. According to the Marburg Toolbox, the part is designed as a 4-part (CDS).

If there is no need for tagging, use this part:

GGTCTCGAATG-coding_region-GCTTTGAGACC

Source

pMMB-tfox vc

https://www.ncbi.nlm.nih.gov/pubmed/28571309

References

https://www.ncbi.nlm.nih.gov/pubmed/28571309